Lectin microarrays identify cell-specific and functionally significant cell surface glycan markers

Lectin microarrays identify cell-specific and functionally significant cell surface glycan markers.

Glycosylation is among the most complex posttranslational modifications with an extremely high level of diversity that has made it refractory to high-throughput analyses. Despite its resistance to high-throughput techniques, glycosylation is important in many critical cellular processes that necessitate a productive approach to their analysis. To facilitate studies in glycosylation, we develope...

Intact cell adhesion to glycan microarrays.

A rapid and reproducible method was developed to detect and quantify carbohydrate-mediated cell adhesion to glycans arrayed on glass slides. Monosaccharides and oligosaccharides were covalently attached to glass slides in 1.7-mm-diameter spots (200 spots/slide) separated by a Teflon gasket. Primary chicken hepatocytes, which constitutively express a C-type lectin that binds to nonreducing termi...

Expression of CK8 and CK17, Specific Epithelial Markers, by Oral Squamous Cell Carcinoma Cell Lines

Objectives Oral squamous cell carcinoma (OSCC) is among the most common cancers worldwide. This study aimed to assess the expression of CK8 and CK17 by OSCC cell lines in Iranian patients. Methods This observational study was conducted on three OSCC cell lines with a minimum of 45 passages. Cells isolated from three patients with OSCC were cultured and passaged. Expression of CK8 and CK17 epi...

Glycan Profiling of Adult T-Cell Leukemia (ATL) Cells with the High Resolution Lectin Microarrays

Cell Surface-Specific N-Glycan Profiling in Breast Cancer

Aberrant changes in specific glycans have been shown to be associated with immunosurveillance, tumorigenesis, tumor progression and metastasis. In this study, the N-glycan profiling of membrane proteins from human breast cancer cell lines and tissues was detected using modified DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE). The N-glycan profiles of membrane...